Ramachandran Map Analysis of the Monomeric Aβ 1-40 and Aβ 1-42 Peptides by Solution NMR
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چکیده
منابع مشابه
Structural Discrimination between Aβ(1-40) and Aβ(1-42) Peptides in Films with Vibrational Circular Dichroism Spectroscopy.
The secondary structure of full-length Aβ(1-40) and Aβ(1-42) peptides in films has been investigated with IR and vibrational circular dichroism (VCD) spectroscopy. From IR spectra, it is shown that the prepared films of Aβ(1-40) and Aβ(1-42) mainly comprise the β-sheet conformation that is characteristic of aggregated and fibrous Aβ. In the VCD spectra, the Aβ(1-42) film shows an intense and sh...
متن کاملInteraction between soluble Aβ-(1-40) monomer and Aβ-(1-42) fibrils probed by paramagnetic relaxation enhancement.
The most common isoforms of amyloid-β (Aβ) proteins are composed of 40 or 42 amino acid residues. While Aβ-(1-40) is the predominant species, Aβ-(1-42) is more fibrillogenic and neurotoxic, suggesting that Aβ-(1-42) plays a critical role in the initiation of amyloid fibril formation. We investigated the mechanisms by which soluble Aβ-(1-40) associates with preformed Aβ-(1-42) seeds. A paramagne...
متن کاملمدل ساز ی به روش شبیه سازی دینامیک مولکولی مونومر Aβ (1-42) و Aβ (1-40) ، به منظور مقایسه نقش آن‌ها در ایجاد بیماری آلزایمر
سابقه و هدف: مطالعه های آسیب‌شناختی، تشکیل پلاک‌های فیبری و توده‌های تجمع یافته پپتید آمیلوئید بتا (Aβ) را یکی از دلایل بیماری آلزایمر پیشنهاد کرده‌اند. از آنجا که این بیماری وابسته به ساختار فضایی پروتئین است در این مقاله به روش شبیه سازی دینامیک مولکولی مراحل آغازین تغییرهای کنفورماسیون دو مونومر از Aβ، Aβ (1-42) و Aβ (1-40) مورد بررسی قرار میگیرد. مواد و روش ها: ساختار NMR دو پپتید Aβ (...
متن کاملMonomeric Aβ1–40 and Aβ1–42 Peptides in Solution Adopt Very Similar Ramachandran Map Distributions That Closely Resemble Random Coil
The pathogenesis of Alzheimer's disease is characterized by the aggregation and fibrillation of amyloid peptides Aβ(1-40) and Aβ(1-42) into amyloid plaques. Despite strong potential therapeutic interest, the structural pathways associated with the conversion of monomeric Aβ peptides into oligomeric species remain largely unknown. In particular, the higher aggregation propensity and associated t...
متن کاملDirect Observations of Amyloid β Self-Assembly in Live Cells Provide Insights into Differences in the Kinetics of Aβ(1–40) and Aβ(1–42) Aggregation
Insight into how amyloid β (Aβ) aggregation occurs in vivo is vital for understanding the molecular pathways that underlie Alzheimer's disease and requires new techniques that provide detailed kinetic and mechanistic information. Using noninvasive fluorescence lifetime recordings, we imaged the formation of Aβ(1-40) and Aβ(1-42) aggregates in live cells. For both peptides, the cellular uptake v...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2017
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2016.11.2605